Premium
The assessment of the robustness of micro RNA s from oral cytological scrapings
Author(s) -
Prasad Gareema,
Seers Christine,
Reynolds Eric,
McCullough Michael J.
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12489
Subject(s) - rna , curette , biopsy , pathology , lesion , carcinoma , medicine , biology , gene , biochemistry
Background Sampling of suspect oral lesions in the general dental clinic may increase early carcinoma detection thus oral cancer survival rates. One means of lesion sampling that is an alternative to incisional biopsy is cytological scraping. Micro RNA alterations are also being explored as a means of diagnosing carcinoma as an alternative to histopathology. Methods We obtained cytological scrapings using 10 strokes (‘light’) or 40 strokes (‘heavy’) from the buccal mucosa of one healthy subject using a dermatological curette. Micro RNA was isolated from oral cytological scrapings immediately, or the scrapings were stored in buffer or RNA later, at 4°C, room temperature or 36°C, from 1 to 7 days prior to RNA isolation. All scrape comparisons and test conditions were conducted in triplicate. Micro RNA s were measured using qRT – PCR . Results Micro RNA s can be obtained from cytological scrapings independent of the number of strokes and can be measured using qRT – PCR after storage under all conditions tested. Conclusion Micro RNA s are robust to a wide range of storage conditions that bodes well for use of cytological scrapings to be of use in a clinical setting as a chair side sampling method for suspect oral lesions.