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Downregulation of nucleophosmin expression inhibited proliferation and induced apoptosis in salivary gland adenoid cystic carcinoma
Author(s) -
Li Shihao,
Zhang Xinyu,
Zhou Zhechong,
Huang Zixian,
Liu Liu,
Huang Zhiquan
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12482
Subject(s) - adenoid cystic carcinoma , salivary gland , downregulation and upregulation , perineural invasion , pathology , nucleophosmin , cancer research , apoptosis , biology , gene knockdown , cell growth , immunohistochemistry , metastasis , carcinoma , medicine , cancer , biochemistry , genetics , myeloid leukemia , gene
Background This study aimed to explore the relationship between nucleophosmin ( NPM 1) and patient clinical characteristics. Moreover, we investigated the effect of NPM 1 in tumor proliferation and apoptosis of salivary gland adenoid cystic carcinoma ( SACC ). Materials and methods NPM 1 expression was examined in 74 specimens of SACC and 31 non‐cancerous epithelium adjacent to carcinoma ( NCEAC ) by immunohistochemistry ( IHC ). RNA interference technology was used to silence NPM 1 expression in SACC cells. We used transwell culture assay, cell counting kit‐8 tests, and colony formation assay to test the proliferation, cisplatin resistance, migration, and invasiveness of SACC cells. Results The nuclear and cytoplasmic expression of NPM 1 in SACC tissue was overexpressed and was tightly linked to perineural invasion and lymph node metastasis. The downregulation of NPM 1 inhibited proliferation and induced apoptosis in SACC cells. Knockdown of NPM 1 expression had no effect on chemoresistance migration, or invasiveness. Conclusions NPM 1 may play an important role in tumor progress in SACC and is a potential biomarker for SACC .