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Expression of podoplanin and TGF ‐beta in glandular odontogenic cyst and its comparison with developmental and inflammatory odontogenic cystic lesions
Author(s) -
Alaeddini Mojgan,
Eshghyar Nosratollah,
EtemadMoghadam Shahroo
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12475
Subject(s) - podoplanin , immunohistochemistry , pathology , radicular cyst , cyst , ameloblastoma , medicine , dentigerous cyst , biology , anatomy , maxilla
Background The number of studies investigating the immunohistochemical characteristics of glandular odontogenic cysts ( GOC s) is limited, due to its rarity. TGF ‐beta has been suggested to induce podoplanin expression in some lesions. We aimed to evaluate and compare podoplanin and TGF ‐beta expression in GOC and other odontogenic cystic lesions. Methods A total of 43 samples including five GOC s, 10 dentigerous cysts ( DC s), eight unicystic ameloblastoma ( UA s), and 20 radicular cysts ( RC s) were selected and subjected to immunohistochemical staining using monoclonal antibodies against podoplanin and TGF ‐beta. Kruskal–Wallis test and Mann–Whitney U ‐test were used for statistical analysis along with Bonferroni for adjusting P ‐values ( P < 0.05). Results Podoplanin immunoreactivity was observed in 80%, 70%, and 100% of DC s, RC s, and UA s, respectively, while none of the GOC s were positive for this marker ( P = 0.004). Significant differences were only found in the GOC specimens. TGF ‐beta positivity occurred in the capsule and epithelium of all GOC s and DC s, while RC s and UA s demonstrated different expression percentages in the capsular and epithelial tissues. Epithelial TGF ‐beta showed significant differences among the studied lesions ( P = 0.007) with the main difference found between DC s with RC s and DC s with UA s. Conclusions Lack of podoplanin expression might be involved in the characteristic histologic and behavioral features of GOC , which seems to be unrelated to TGF ‐beta expression.