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CDH 11 inhibits proliferation and invasion in head and neck cancer
Author(s) -
Piao Songlin,
Inglehart Ronald C.,
Scanlon Christina Springstead,
Russo Nickole,
Banerjee Rajat,
D'Silva Nisha J.
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12471
Subject(s) - head and neck squamous cell carcinoma , gene knockdown , cancer research , cell growth , downregulation and upregulation , biology , cancer , tissue microarray , pathology , immunohistochemistry , microarray analysis techniques , cell , cell culture , in silico , head and neck cancer , medicine , gene expression , gene , biochemistry , genetics
Background In this study, we use a bioinformatics‐based strategy to nominate a tumor suppressor gene cadherin‐11 ( CDH 11) and investigate its role in growth and invasion in head and neck squamous cell carcinoma ( HNSCC ). Methods Using the Oncomine ™ database to compare HNSCC and normal specimens, CDH 11 was nominated as having a role in HNSCC . CDH 11 expression in HNSCC was evaluated by immunohistochemistry on a tissue microarray ( TMA ) and immunoblotting and immunofluorescence of cell lines. The functional impact of CDH 11 on proliferation and invasion was evaluated after si RNA ‐mediated knockdown. Results In silico analysis suggested that CDH 11 is overexpressed in HNSCC compared to normal specimens. HNSCC TMA exhibited a small but significant increase in intensity and proportion of CDH 11. By immunoblot analysis, CDH 11 was higher in 4/7 HNSCC cell lines compared to normal keratinocytes; CDH 11 was highly upregulated in UM ‐ SCC ‐47 and UM ‐ SCC ‐74A and detectable in UM ‐ SCC ‐14A and UM ‐ SCC ‐29 cell lines. Downregulation of CDH 11 in both UM ‐ SCC ‐29 and UM ‐ SCC ‐47 using two different si RNA s enhanced proliferation and invasion. Conclusion CDH 11 inhibits cell proliferation and invasion of HNSCC . This suggests that CDH 11 functions as a tumor suppressor gene in head and neck cancer. Our findings emphasize the importance of verifying in silico findings with functional studies.