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Evaluation of diagnostic value of Ag NOR and PAP in early detection of dysplastic changes in leukoplakia and lichen planus – a preliminary case–control study
Author(s) -
Rao Dhanya S.,
Ali I. M.,
Annigeri Rajeshwari G.
Publication year - 2017
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12457
Subject(s) - leukoplakia , papanicolaou stain , medicine , pathology , dysplasia , oral lichen planus , cancer , cervical cancer
Background and Objectives Early detection of oral cancer has been the most effective approach to reduce morbidity and mortality of cancer patients. If a lesion is clinically considered suspicious, an easily practicable, non‐invasive, painless, safe, and accurate screening method for detection of the dysplastic changes is necessary. In an attempt to procure this, a study was conducted with the aim of determining the diagnostic accuracy of rapid Papanicolaou stain ( PAP ) and silver‐stained nucleolar organizer regions (Ag NOR ) in brush biopsies of potentially malignant lesions for early detection of oral cancer. Methodology Brush biopsies taken from 25 cases of leukoplakia and lichen planus each were stained with rapid PAP and silver nitrate stains. Histopathological correlation was performed and further compared with rapid PAP and Ag NOR for its diagnostic validity. Results Statistically significant increase in the mean Ag NOR count was seen from normal epithelium to lichen planus to that of leukoplakia. When compared with rapid PAP , a linear correlation was seen in Ag NOR counts and stages of dysplasia in leukoplakia which was also found to be statistically significant. Diagnostic accuracy for Ag NOR in leukoplakia was found to be 84%, lichen planus 73%, whereas RAPID PAP showed 72% accuracy. Conclusion Ag NOR analysis may be useful as a quantitative marker of incipient cellular alterations and hence would be helpful in assessing suspicious lesions and thus can be regarded as a valuable adjunct.