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PI 3K– AKT – mTOR pathway proteins are differently expressed in oral carcinogenesis
Author(s) -
Martins Fabiana,
Sousa Suzana COM,
Santos Elisa,
Woo SookBin,
Gallottini Marina
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12440
Subject(s) - pi3k/akt/mtor pathway , immunohistochemistry , carcinogenesis , protein kinase b , epithelial dysplasia , pathology , staining , cancer research , medicine , cancer , biology , signal transduction , microbiology and biotechnology
Background PI 3K– AKT – mTOR signaling pathway is associated with several cellular functions and is frequently changed in several malignancies. The aim of this study was to characterize the immunohistochemical expression pattern of components in PI 3K– AKT – mTOR signaling pathway in oral epithelial dysplasia ( OED ), comparing to oral squamous cell carcinoma ( OSCC ) and non‐dysplastic oral tissues ( NDOT ). Methods A total of 186 cases of NDOT , OED and OSCC were retrieved. Nuclear staining and cytoplasmic staining of the keratinocytes were considered positive, and the percentage of positive cells was calculated. Results Increased immunoreactivity from NDOT to OED and OSCC was seen for all proteins. In NDOT cases, positivity was found only for pS 6 (52.9%) and p4 EBP 1 (13.5%). In OED , immunoreactivity was observed for pAKT (62.2%), pm TOR (28.6%), pS 6 (70.8%), and p4 EBP 1 (42.9%). In OSCC cases, immunoreactivity was found for pAKT (83.3%), pm TOR (50%), pS 6 (77.4%), and p4 EBP 1 (50%). The pAKT and pm TOR expression was higher in OED (<0.001, Fisher's exact test) and OSCC (<0.001, Fisher's exact test). Conclusion Our study demonstrated higher pAKT and pm TOR expression during carcinogenesis of oral mucosa, differing considerably among OED and OSCC specimens when compared to NDOT. These proteins can be considered potential diagnostic markers for early detection of cancer.