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Use of photodynamic therapy and chitosan for inactivacion of Candida albicans in a murine model.
Author(s) -
Fabio CamachoAlonso,
Yolanda MartínezBeneyto,
Carmen Gallego Mª,
Francisco Cuello,
Antonio Julián Buendía,
Leonor PérezLajarin,
Jesús Salinas
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12435
Subject(s) - candida albicans , chitosan , photodynamic therapy , nystatin , methylene blue , corpus albicans , inoculation , microbiology and biotechnology , photosensitizer , medicine , fungicide , antifungal , chemistry , biology , immunology , biochemistry , organic chemistry , photocatalysis , catalysis , botany
Background The wide use of topical and systemic antifungal agents as the conventional treatment for oral candidiasis has caused Candida albicans to develop resistance to these agents. The aims of this study were to evaluate the effects of photodynamic therapy ( PDT ) and chitosan on buccal candidiasis and study the possible enhancive effect of chitosan on the photosensitizer methylene blue. Methods Thirty‐five DBA /2 immunosuppressed mice were orally inoculated with a suspension of Candida albicans . The animals were randomized into seven groups ( n = 5 per group): Group 1 (control); Group 2 (nystatin); Group 3 ( PDT ); Group 4 (chitosan 1.5 mg/ml); Group 5 (chitosan 3 mg/ml); Group 6 ( PDT + chitosan 1.5 mg/ml); and Group 7 ( PDT + chitosan 3 mg/ml). The Candida albicans count was evaluated on days 3, 5, 7, and 11 after inoculation. At last, macroscopic and microscopic analyses of the tongue dorsa were performed. Results On day 7 after inoculation, the control group showed a greater number of Candida albicans (5.25 ± 0.41 log 10 CFU /ml), with significant differences compared to all other groups ( P ≤ 0.05). On day 11 after inoculation, animals treated with PDT showed lower CFU /ml count. Groups 2, 3, 4, 5, and 6 showed fewer microscopic candidiasis lesions than Groups 1 and 7. Conclusions PDT has an antifungal effect, even greater than nystatin. Chitosan has a powerful fungicide effect but did not possess any enhancive effect on methylene blue.