Assessment of salivary total antioxidant capacity in patients with primary untreated head and neck squamous cell carcinoma with ORAC
Author(s) -
Wesołowski Piotr,
Zawada Katarzyna,
Wojtowicz Andrzej,
Strużycka Izabela,
Kamiński Tomasz
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12433
Subject(s) - oxygen radical absorbance capacity , oxidative stress , saliva , antioxidant , head and neck squamous cell carcinoma , grading (engineering) , antioxidant capacity , medicine , gastroenterology , head and neck cancer , pathology , chemistry , biochemistry , biology , cancer , ecology
Background Oxidative DNA damage leads to abnormalities in endogenous cellular processes and constitutes a direct link between free radicals, antioxidants and squamous cell carcinoma ( SCC ). Oxygen radical absorbance capacity ( ORAC ) method is used to measure antioxidant capacity versus oxygen free radicals. There is no report on the application of ORAC to evaluate salivary antioxidant capacity in patients with head and neck SCC . This study investigated the relation between total antioxidant capacity of saliva, measured with ORAC method, and the incidence, location, size and histopathological grading of SCC . Methods Salivary antioxidant capacity was assessed in 30 male and female patients with histopathologically confirmed oral and laryngeal SCC . Histopathological grading of SCC was based on WHO classification. Results Mean ORAC in patients with SCC was higher than the mean value in the control group. Statistically significant difference was observed in the study group versus the control group. No significant differences were observed for ORAC values in relation to histopathological grading, tumour size and spread to lymph nodes. Conclusions The obtained results can advocate local compensatory mechanism in saliva in response to increased oxidative stress associated with primary tumour. ORAC can become an additional factor in head and neck SCC prognosis, but further investigation is required.