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IL ‐1β‐regulating angiogenic factors expression in perforated temporomandibular disk cells via NF ‐κB pathway
Author(s) -
Xu Jie,
Cai Hengxing,
Meng Qinggong,
Li Yingjie,
Chen Guoxin,
Fang Wei,
Long Xing
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12420
Subject(s) - angiogenesis , western blot , angiogenin , vascular endothelial growth factor , p38 mitogen activated protein kinases , umbilical vein , chemistry , matrix metalloproteinase , signal transduction , microbiology and biotechnology , biology , mapk/erk pathway , cancer research , vegf receptors , in vitro , biochemistry , gene
Background A high density of blood vessels is observed in the perforated disks of temporomandibular joint ( TMJ ), but the underlying mechanism is unknown. This study aimed to explore the regulation of disk angiogenesis in the perforated disks. Methods Expressions of vascular endothelial growth factor ( VEGF ), angiogenin‐1 (Ang‐1), chondromodulin‐1 (ChM‐1), and thrombospondins‐1 ( TSP ‐1) were compared between healthy and perforated TMJ disk cells with or without interleukin‐1β ( IL ‐1β) incubation. The tube formation, cell migration, and expressions of matrix‐metalloproteinases ( MMP s) in human umbilical vein endothelial cell line ( HUV ‐ EC ‐C) were investigated in conditional media of disk cells. Western blot was performed to determine protein level of VEGF , Ang‐1, ChM‐1 and TSP ‐1 in IL ‐1β‐induced disk cells cultured by NF ‐κB‐ or P38‐specific pathway inhibitors, respectively. Results Conditional media from perforated disk cells induced more tube formation, cell migration, and MMP s’ expression in HUV ‐ EC ‐C. Expressions of VEGF and Ang‐1 were significantly higher, and ChM‐1 and TSP ‐1 were lower in perforated disks compared to healthy disks. The VEGFA concentration was 291.1 ± 36.09 pg/ml in perforated disk cell conditioned media, markedly larger than that in NDCCM (144.9 ± 33.69 pg/ml). IL ‐1β induced VEGF through NF ‐κB signaling pathway and Ang‐1 through p38 MAPK pathway, while repressed expression of ChM‐1 and TSP ‐1 was through NF ‐κB pathway. Blockade of each pathway markedly restrained inducing effect of cultural media on HUV ‐ EC ‐C tube formation and migration. Conclusions Perforated disk cells secreted more angiogenic factors which might induced via NF ‐κB pathway.