z-logo
Premium
Overexpression of Bcl‐2, SOCS 1, 3 and Cdh 1, 2 are associated with the early neoplasic changes in modified 4‐nitroquinoline 1‐oxide‐induced murine oral cancer model
Author(s) -
Cabrera Ortega Adriana Alicia,
Gonçalves Vinícius de Paiva,
Guimarães Morgana Rodrigues,
Rossa Junior Carlos,
Spolidorio Luis Carlos
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12413
Subject(s) - 4 nitroquinoline 1 oxide , proliferating cell nuclear antigen , vimentin , immunohistochemistry , epithelial dysplasia , dysplasia , pathology , carcinogenesis , medicine , cancer , cancer research , biology
Background The objective was to assess histopathological changes and the expression of proliferating cell nuclear antigen ( PCNA ), Bcl‐2, suppressor of cytokine signaling ( SOCS ) 1 and 3, Vimentin, TWIST 1, and Cdh 1 and 2 in early stages of experimental oral carcinogenesis process using a shorter period of exposure to 4‐nitroquinoline oxide (4‐ NQO ) model. Methods In this study, 20 rats were divided into control group ( n = 10), sacrificed on the first day of the experiment, and experimental group ( n = 10) treated with 50 ppm of 4‐ NQO solution dissolved in drinking water for 8 and 12 weeks. The histological sections were stained with H&E or subjected to immunohistochemistry for detecting PCNA , Bcl‐2, SOCS 1 and 3, and STAT 3. Some specimens were used for verification of Vimentin expression, Cdh 1, Cdh 2, and TWIST 1 by RT – qPCR . Results At both 8 and 12 weeks, morphological changes occurred mainly in the posterior portion of the tongue and were limited to the epithelial tissue, including moderate to severe dysplasia at 8 weeks, and severe dysplasia with exacerbation of atypical cells at 12 weeks. Expression of SOCS 1 and 3 increased from 8 to 12 weeks ( P < 0.05), whereas STAT 3 expression was reduced mainly at 12 weeks ( P < 0.05) in comparison with the control group. The expression of all epithelial–mesenchymal transition markers ( EMT ) was increased after 12 weeks, reaching statistical significance ( P < 0.05) for Cdh 1 and 2. Conclusions Together, the results suggested that overexpression of Bcl‐2, SOCS 1 and 3, and Cdh 1 and 2 is associated with the early neoplasic changes in modified 4‐nitroquinoline 1‐oxide‐induced murine oral cancer model.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here