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Interaction of stromal and microvascular components in keratocystic odontogenic tumors
Author(s) -
SousaNeto Ernesto Santos,
Cangussu Maria Cristina Teixeira,
Gurgel Clarissa Araújo,
Guimarães Vanessa Sousa,
Ramos Eduardo Antônio Gonçalves,
Xavier Flávia Caló Aquino,
Cury Patrícia Ramos,
Carneiro Júnior Braúlio,
Leonardi Rosalia,
Dos Santos Jean Nunes
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12400
Subject(s) - cd34 , stromal cell , tryptase , mast cell , angiogenesis , chemistry , pathology , cd163 , immunohistochemistry , lymphatic vessel , lymphangiogenesis , cancer research , medicine , immunology , biology , microbiology and biotechnology , cancer , metastasis , biochemistry , stem cell , gene , phenotype
Objective Little is known about the interaction of stromal components in odontogenic tumors. Thus, the aim of this study was to investigate mast cells ( MC s), myofibroblasts, macrophages, and their possible association with angiogenesis and lymphangiogenesis in keratocystic odontogenic tumors ( KCOT s). Material and Methods Thirty cases of KCOT s were included and analyzed by immunohistochemistry for mast cell tryptase, α‐ SMA , CD 34, CD 163, and D240. For comparative purpose, 15 radicular cysts ( CR s) and 7 pericoronal follicles ( PF s) were included. Results There was an increase in MC s for RC s and this difference was significant when they were compared to KCOTS and PF s. A significant increase in the density of MF s was observed for KCOT s when compared to RC s and PF s ( P = 0.00). No significant difference in CD 163‐positive macrophages ( P = 0.084) and CD 34‐positive vessels ( P = 0.244) densities was observed between KCOT s, RC s, and PF s, although KCOT s showed a higher density of all proteins. Significant difference in lymphatic vessel density was observed for KCOT s when compared to RC s and PF s ( P = 0.00). Positive correlation was observed between mast cell tryptase and CD 34 in KCOT s ( P = 0.025). Conclusions A significant interaction between the MC population and CD 34‐positive vessels in KCOT s supported the hypothesis that MC s and blood vessels contribute to the stromal scaffold of KCOT .