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Copy number increase of oncoprotein CIP 2A is associated with poor patient survival in human head and neck squamous cell carcinoma
Author(s) -
Routila Johannes,
Bilgen Türker,
Saramäki Outi,
Grénman Reidar,
Visakorpi Tapio,
Westermarck Jukka,
Ventelä Sami
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12372
Subject(s) - head and neck squamous cell carcinoma , immunohistochemistry , tissue microarray , biomarker , cancer research , copy number variation , biology , head and neck cancer , fluorescence in situ hybridization , carcinoma , cancer , oncology , pathology , medicine , gene , genetics , genome , chromosome
Background CIP 2A, an inhibitor of PP 2A tumour suppressor function, is a widely overexpressed biomarker of aggressive disease and poor therapy response in multiple human cancer types. Methods CIP 2A and DPPA 4 copy number alterations and expression were analysed by fluorescence in situ hybridisation ( FISH ) and immunohistochemistry ( IHC ) in different cell lines and a tissue microarray of 52 HNSCC patients. Results were correlated with patient survival and other clinicopathological data. Results CIP 2A and DPPA 4 copy number increase occurred at a relatively high frequency in human HNSCC patient samples. CIP 2A but not DPPA 4 FISH status was significantly associated with patient survival. CIP 2A detection by combining IHC with FISH yielded superior resolution in the prognostication of HNSCC . Conclusions CIP 2A copy number increase is associated with poor patient survival in human HNSCC . We suggest that the reliability and prognostic value of CIP 2A detection can be improved by performing FISH analysis to CIP 2A IHC positive tumours.