Premium
Micro RNA ‐155‐5p is associated with oral squamous cell carcinoma metastasis and poor prognosis
Author(s) -
Baba Osamu,
Hasegawa Shogo,
Nagai Hiroki,
Uchida Fumihiko,
Yamatoji Masanobu,
Kanno Naomi I.,
Yamagata Kenji,
Sakai Satoshi,
Yanagawa Toru,
Bukawa Hiroki
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12351
Subject(s) - metastasis , microrna , epithelial–mesenchymal transition , transfection , cancer research , biology , gentamicin protection assay , pathology , cell culture , cancer , medicine , gene , biochemistry , genetics
Background Abnormal mi RNA expression was recently implicated in the metastasis of oral squamous cell carcinoma ( OSCC ) and with a poor prognosis. The initiation of the invasion‐metastasis cascade involves epithelial‐mesenchymal transition ( EMT ). Our aim was to clarify how mi RNA , especially miR‐155‐5p misexpression contributes to OSCC metastasis through EMT . Methods We collected tumor samples from 73 subjects with OSCC . The samples were analyzed by quantitative reverse‐transcription polymerase chain reaction ( qRT ‐ PCR ), and correlations between miR‐155‐5p levels and clinical characteristics were investigated. OSCC cell lines were analyzed by mi RNA microarray and by transfection with a miR‐155‐5p mimic or inhibitor, followed by proliferation and wound‐healing migration assays. qRT ‐ PCR analyses of EMT makers in cells transfected with miR‐155‐5p inhibitor were performed. Results We found high miR‐155‐5p expression in tissue samples from subjects with OSCC that had metastasized to cervical lymph nodes. HSC ‐3 cells also strongly expressed miR‐155‐5p. The epithelial marker E‐cadherin was strongly expressed in HSC ‐3 cells transfected with miR‐155‐5p inhibitor, and we observed elevated SOCS 1 and decreased STAT 3 expression in these cells. Conclusions Our results suggest that miR‐155‐5p causes OSCC to metastasize, and could serve as a novel therapeutic target for OSCC .