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Semaphorins and neuropilins expression in salivary gland tumors
Author(s) -
Fonseca Felipe P.,
Bingle Lynne,
SantosSilva Alan R.,
Lopes Márcio A.,
Almeida Oslei P.,
Andrade Bruno A. B.,
Mariano Fernanda V.,
Kowalski Luiz P.,
Rangel Ana L. C. A.,
Martins Manoela D.,
Meurer Luise,
Speight Paul M.,
Vargas Pablo A.
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12341
Subject(s) - semaphorin , sema3a , immunohistochemistry , plexin , salivary gland , pathology , cd34 , biology , tissue microarray , pathogenesis , medicine , receptor , microbiology and biotechnology , stem cell
Background Salivary gland tumors ( SGT ) account for 3–10% of all head and neck neoplasms, and little is known about their angiogenic properties. Despite semaphorins and neuropilins have been demonstrated to be prognostic determinants in many human cancers, they remain to be investigated in SGT . Therefore, the objective of this study was to analyze the clinical significance of the expression of class 3 semaphorins A (Sema3A) and B (Sema3B) and neuropilins‐1 (Np‐1) and neuropilins‐2 (Np‐2), in SGT . Methods Two hundred and forty‐eight SGT were organized in tissue microarray paraffin blocks and expression of CD 34, Sema3A, Sema3B, Np‐1, and Np‐2 was determined through immunohistochemistry. The immunoreactions were quantified using digital algorithms and the results correlated with clinicopathological parameters. Results Malignant tumors had an increased vascular density than their benign counterparts and their increased vascular area significantly correlated with recurrences ( P < 0.05). Patients older than 40 years and the presence of recurrences determined an inferior survival rate ( P = 0.0057 and P = 0.0303, respectively). In normal salivary glands, Np‐1 and Np‐2 expression was restricted to ductal cells, whereas Sema3A and Sema3B were positive in the serous acinar compartment. Tumors were positive for all markers and the co‐expression of Np‐1/Np‐2 significantly correlated with the presence of paresthesia and advanced stages of the tumors ( P = 0.01 and P = 0.04, respectively). Conclusion Sema3A, Sema3B, Np‐1, and Np‐2 may be involved in the pathogenesis of SGT , but their expression did not present a statistically significant prognostic potential in this study.