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The expression of high‐mobility group box protein‐1 in temporomandibular joint osteoarthritis with disc perforation
Author(s) -
Feng Yaping,
Fang Wei,
Li Cheng,
Guo Huilin,
Li Yingjie,
Long Xing
Publication year - 2016
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12336
Subject(s) - temporomandibular joint , perforation , medicine , osteoarthritis , blot , immunohistochemistry , downregulation and upregulation , inflammation , pathology , chemistry , materials science , gene , biochemistry , alternative medicine , punching , metallurgy
Background High‐mobility group box protein‐1 ( HMGB ‐1), a potent promoter of inflammation, was believed to be a potential trigger of osteoarthritis ( OA ). Only a few studies have investigated the role of HMGB ‐1 in temporomandibular joint ( TMJ ) OA , especially in disc perforation cases. But in this study, not only the expression of HMGB ‐1 in TMJ OA with disc perforation was investigated but also the possible role of HMGB ‐1 in TMJ OA was discussed. Methods Synovial membrane and disc specimens were collected from patients with TMJ OA , and the expression of HMGB ‐1 was detected using immunohistochemistry, real‐time quantitative PCR and Western blotting. Results High‐mobility group box protein‐1 expressed strongly in cytoplasm and nucleus of lining layer cells and endothelial cells in osteoarthritic synovium. Staining of HMGB ‐1 was found intensive in the frontier tissue of the perforation in the perforated discs. HMGB ‐1 expression was also upregulated in osteoarthritic synovial cells and disc cells according to real‐time quantitative PCR and Western blotting analysis. Conclusions High‐mobility group box protein‐1 expression was upregulated in TMJ OA and might promote the progression of TMJ OA .