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Resveratrol suppresses TPA ‐induced matrix metalloproteinase‐9 expression through the inhibition of MAPK pathways in oral cancer cells
Author(s) -
Lin FengYan,
Hsieh YiHsien,
Yang ShunFa,
Chen ChangTai,
Tang ChihHsin,
Chou MingYung,
Chuang YiTing,
Lin ChiaoWen,
Chen MuKuan
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12288
Subject(s) - resveratrol , viability assay , mapk/erk pathway , zymography , chemistry , cell migration , kinase , cancer cell , microbiology and biotechnology , protein kinase a , cancer research , cell , matrix metalloproteinase , pharmacology , biology , cancer , biochemistry , genetics
Background Naturally occurring agents, such as resveratrol, have been determined to benefit health. Numerous studies have demonstrated that resveratrol has antioxidative, cardioprotective, and neuroprotective properties. However, the effect of resveratrol exerts on the metastasis of oral cancer cells remains unclear. In this study, we investigated the effect the anti‐invasive activity of resveratrol on a human oral cancer cell line ( SCC ‐9) in vitro and the underlying mechanisms. Methods Cell viability was examined by MTT assay, whereas cell motility was measured by migration and wound‐healing assays. Zymography, reverse‐transcriptase polymerase chain reaction ( PCR ), and promoter assays confirmed the inhibitory effects of resveratrol on matrix metalloproteinase‐9 ( MMP ‐9) expression in oral cancer cells. Results We established that various concentrations (0–100 μM) of resveratrol inhibited the 12‐O‐tetradecanoylphorbol‐13‐acetate ( TPA )‐induced migration capacities of SCC ‐9 cells and caused no cytotoxic effects. Zymography and Western blot analyses suggested that resveratrol inhibited TPA ‐induced MMP ‐9 gelatinolytic activity and protein expression. In addition, the results indicated that resveratrol inhibited the phosphorylation of c‐Jun N‐terminal kinase ( JNK )1/2 and extracellular‐signal‐regulated kinase ( ERK )1/2 involved in downregulating protein expression and the transcription of MMP ‐9. Conclusion In summary, resveratrol inhibited MMP ‐9 expression and oral cancer cell metastasis by downregulating JNK 1/2 and ERK 1/2 signals pathways and, thus, exerts beneficial effects in chemoprevention.