Premium
Lipopolysaccharide enhances OSCC migration by promoting epithelial–mesenchymal transition
Author(s) -
He Zhifeng,
Deng Runzhi,
Huang Xiaofeng,
Ni Yanhong,
Yang Xudong,
Wang Zhiyong,
Hu Qingang
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12285
Subject(s) - epithelial–mesenchymal transition , vimentin , cell migration , mesenchymal stem cell , lipopolysaccharide , western blot , cell , wound healing , cell culture , chemistry , cancer research , microbiology and biotechnology , biology , immunology , metastasis , immunohistochemistry , cancer , biochemistry , genetics , gene
Background This study was performed to examine whether lipopolysaccharide can influence cell migration and epithelial–mesenchymal transition of oral squamous cell carcinoma. Methods Three oral squamous cell carcinoma cell lines ( HSC 3, CAL 27, and SCC 4) were obtained for the study. TLR 4 expression in three cell lines was analyzed by Q‐ PCR and Western blot. After cells treated with LPS , cell migration was analyzed by wound‐healing and chemotaxis cell migration assay. Changes of E ‐cadherin and vimentin expression were tested by Western blot and immunofluorescence staining. To examine NF ‐κB activation, NF ‐κB nuclear translocation was investigated. Results TLR 4 was expressed in all three cell lines and was highest in HSC 3 while lowest in SCC 4. TLR 4 ligand lipopolysaccharide accelerated wound healing and enhanced cell migration. Also, it stimulated epithelial–mesenchymal transition demonstrated by decreased E ‐cadherin and increased vimentin expression. Lipopolysaccharide also provoked NF ‐κB nuclear translocation. Either TLR 4 or NF ‐κB blocking reverted these effects. Conclusions Lipopolysaccharide can induce TLR 4‐mediated epithelial–mesenchymal transition and cell migration in oral squamous cell carcinoma. These responses could further affect tumor progressing by inducing tumor cell metastasis.