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Macrophage‐mediated nanoparticle delivery to the periodontal lesions in established murine model via Pg‐ LPS induction
Author(s) -
Ma Zhiwei,
DagnæsHansen Frederik,
Løvschall Henrik,
Song Wen,
Nielsen Gitte K.,
Yang Chuanxu,
Wang Qintao,
Kjems Jørgen,
Gao Shan
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12269
Subject(s) - porphyromonas gingivalis , periodontitis , lipopolysaccharide , macrophage , pathology , in vivo , gingival sulcus , inflammation , chitosan , infiltration (hvac) , chemistry , cellular infiltration , medicine , in vitro , immunology , dentistry , biology , materials science , biochemistry , microbiology and biotechnology , composite material
We established a murine periodontitis model by local injection of lipopolysaccharide of Porphyromonas gingivalis (Pg‐ LPS ) into the gingival sulcus of mandibular left incisor four times with 48‐h interval. The histological examination of the periodontal tissues demonstrated that significant loss of periodontal bone and ligaments was observed in the lesion side with abundant inflammatory cell infiltration. Two days after the last injection, Cy5‐labelled si RNA /chitosan particles were injected intraperitoneally (ip). The chitosan/si RNA particles were taken up by peritoneal macrophages, which subsequently migrated to the inflamed gingival area evaluated by in vivo imaging. The localization of macrophages in the inflamed region was further confirmed by immunofluorescent staining. The present report demonstrates that intragingival injection of Pg‐ LPS can be used to create an experimental model of periodontal inflammation in mice and that recruitment of macrophages with chitosan/si RNA nanoparticles to the inflamed area opens the possibility of an RNA i‐based therapeutic approach using chitosan as a carrier in periodontitis.