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Proteomics profiling of keratocystic odontogenic tumours reveals AIDA as novel biomarker candidate
Author(s) -
Ivanišević Malčić Ana,
Breen Lucas,
Josić Djuro,
Jukić Krmek Silvana,
Džombeta Tihana,
Matijević Jurica,
Grgurević Lovro,
Pavelić Krešimir,
Krušlin Božo,
Kraljević Pavelić Sandra
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12239
Subject(s) - biology , proteomics , proteome , immunohistochemistry , pathology , keratocyst , biomarker , cancer research , odontogenic , bioinformatics , immunology , medicine , genetics , gene
Background Keratocystic odontogenic tumour ( KCOT ) is a benign, yet aggressive odontogenic tumour. Herein, proteome analysis of KCOT lesions in comparison with control patient‐matched tissue unaffected by the disease and with inflammatory odontogenic cysts, namely radicular cysts is presented. Methods For the proteomics profiling, two complementary proteomics techniques MALDI ‐ MS / MS and LC ‐ ESI ‐ MS / MS were employed. Potential candidate biomarkers were validated by immunohistochemistry. Results More than 43 proteins were found to be differentially expressed or up‐regulated in KCOT lesions in comparison with patient‐matched unaffected oral mucosa. These proteins bear important biological functions and are involved in cell proliferation, cytoskeletal re‐organization, transcription, cellular motility and apoptosis. In particular, a number of differentially expressed proteins participate in autocrine regulation and signalization within JNK and p38 MAPK signalling pathways. Conclusions Immunohistochemical validation of chosen putative biomarkers revealed axin interaction partner and dorsalization‐antagonist ( AIDA ), known as a protein that blocks activation of JNK signalling pathway, as a differential biomarker for KCOT lesions on an independent cohort of KCOT tissue samples in comparison with most prevalent intra‐oseal lesions inflammatory odontogenic cysts.