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Hyperthermia inhibited the migration of tongue squamous cell carcinoma through TWIST 2
Author(s) -
Tang Yaling,
Jiang Jian,
Liu Jeffrey,
Zheng Min,
He Yongwen,
Chen Wei,
Fan Yunlong,
Chen Qianming,
Liao Chuhang,
Liang Xinhua
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12237
Subject(s) - hyperthermia , cancer research , metastasis , cell migration , epithelial–mesenchymal transition , transfection , cell , cell culture , cell growth , tumor progression , head and neck squamous cell carcinoma , pathology , medicine , biology , cancer , head and neck cancer , genetics
Background Hyperthermia has been shown promising in the treatment of head and neck squamous cell carcinoma ( HNSCC ); however, the mechanism underlying hyperthermia reducing tumor metastasis is poorly elucidated. TWIST 2, an important transcription factor of epithelial‐mesenchymal transition ( EMT ), plays a critical role in the tumor progression and metastasis. The role of TWIST 2 in tongue squamous cell carcinoma ( TSCC ) and its association with hyperthermia still have not been reported. Method The correlations between TWIST 2 expression and the clinical‐pathologic characteristics of 89 patients with TSCC were evaluated by immunohistochemical staining. TSCC cell lines transfected with si RNA against TWIST 2 were heated for 40 min at 42.5°C, and the migration capability of cells was examined by migration assay. Xenograft tumors in nude mice were treated by hyperthermia, and TWIST 2 expression was measured. Results Our data showed that TWIST 2 expression was associated with the metastasis of human TSCC . In Tca8113 and Cal‐27 cells, TWIST 2‐si RNA treatment can reduce cell migration ability and has no effect on the cell proliferation and apoptosis. Hyperthermia can decrease the level of TWIST 2 in TSCC and inhibit the migration of cells. Conclusions This demonstrated that hyperthermia might decrease the migration of Tca8113 and Cal‐27 cells by reducing TWIST 2 expression. Altogether, these findings suggest an as yet undescribed link between TWIST 2 and hyperthermia in TSCC .