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Decreased expression of glutathione S‐transferase pi correlates with poorly differentiated grade in patients with oral squamous cell carcinoma
Author(s) -
Ma Hailong,
Yu Cong,
Liu Ying,
Tan Yiran,
Qiao Jinke,
Yang Xi,
Wang Lizhen,
Li Jiang,
Chen Qiong,
Chen Fuxiang,
Zhang Zhiyuan,
Zhong Laiping
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12229
Subject(s) - gstp1 , immunohistochemistry , glutathione s transferase , western blot , pathological , pathology , medicine , glutathione , cancer research , biology , enzyme , biochemistry , gene
Background Glutathione S transferase pi ( GSTP 1) is a member of phase II detoxification enzymes as a major regulator of cell signaling in response to stress, hypoxia, growth factors, and other stimuli. The clinical role of GSTP 1 in cancer is still unclear. The aim of this study was to investigate the serum GSTP 1 level in patients with oral squamous cell carcinoma ( OSCC ) and the GSTP 1 expression in tissue samples from patients with OSCC and OSCC lines. Methods One hundred and sixty‐six patients with OSCC and 120 normal persons were used to screen potential serum peptide biomarkers using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry ( MALDI ‐ TOF MS ). Serum GSTP 1 concentration was detected in 18 patients with OSCC and 18 normal persons using ELISA . Immunohistochemistry was used to detect GSTP 1 expression in tissue samples from twenty‐eight OSCC patients. Western blot and real‐time PCR were used to detect GSTP 1 expression in nine OSCC lines. Results Decreased GSTP1 concentration was found in the patients with OSCC compared with the normal persons by MALDI‐TOF‐MS, which was then confirmed by ELISA ( P = 0.019). Decreased GSTP1 mRNA level and protein expression were also found in the OSCC lines. Decreased GSTP1 expression was found correlating with pathological differentiation grade in the tissue samples from OSCC patients, a lower GSTP1 expression indicating a poorer pathological differentiation grade ( P = 0.041). Conclusions These results suggest that decreased GSTP 1 expression in patients with OSCC and a lower GSTP 1 expression indicating a poorer pathological differentiation grade in OSCC tissue samples.