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High expression of the histone demethylase LSD 1 associates with cancer cell proliferation and unfavorable prognosis in tongue cancer
Author(s) -
Yuan Chunping,
Li Zhongwu,
Qi Bing,
Zhang Wei,
Cheng Jie,
Wang Yanling
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12220
Subject(s) - cancer , tongue , carcinogenesis , malignancy , medicine , cancer research , immunohistochemistry , biomarker , pathology , tongue neoplasm , oncology , biology , biochemistry
Background The histone lysine‐specific demethylase ( LSD 1) is a key chromatin modifier mediating the demethylation of both H3K4me1/me2 and H3K9 me1/me2. Recently, its deregulation has been implicated in the initiation and progression of various cancers. The aim of this study was to investigate the expression pattern of LSD 1 in tongue squamous cell carcinoma ( SCC ) and determine its prognostic significance in predicting patients' prognosis. Methods LSD 1 expression was examined by RT ‐ PCR and western blotting in three tongue cancer cell lines and by immunohistochemistry in 63 primary tongue SCC specimens with detailed clinical, pathological, and follow‐up data. Its associations with various clinicopathological parameters, Ki‐67 expression, and patients' survival were further assessed. Results Upregulated LSD 1 expression was observed in tongue cancer cells and a major fraction of tongue SCC samples. Overexpression of LSD 1 significantly associated with tumor size ( P = 0.0357), pathological grade ( P = 0.0323), Ki‐67 abundance ( P = 0.0148), and reduced overall and disease‐free survival (Kaplan–Meier analysis, P = 0.0351, 0.0479, respectively). The Cox regression survival analyses identified LSD 1 as an important independent predictor for patients' overall survival. Conclusion Our data indicate that aberrant LSD 1 overexpression associates with key clinicopathological features and unfavorable prognosis in patients with tongue cancer. LSD 1 might play critical roles during tongue tumorigenesis and represent a novel biomarker and potential therapeutic target for this malignancy.