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A study on the inhibition of VEGF expression in salivary gland adenoid cystic carcinoma cells via iNOS gene RNAi in vitro
Author(s) -
Ou Yang KeXiong,
Liang Jun,
Yang ZiNan,
Zhao JianJiang
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12217
Subject(s) - adenoid cystic carcinoma , flow cytometry , cell cycle , rna interference , gene silencing , vascular endothelial growth factor , biology , cell culture , apoptosis , cancer research , microbiology and biotechnology , transfection , cell growth , cell , salivary gland , in vitro , carcinoma , gene , vegf receptors , rna , biochemistry , genetics
In this study, we aimed to explore the effect of inducible nitric oxide synthase ( iNOS ) on vascular endothelial growth factor ( VEGF ) expression in salivary gland adenoid cystic carcinoma ( SACC ). Using RNAi , we transfected chemically synthesised iNOS siRNA into ACC ‐M cells (a highly metastatic adenoid cystic carcinoma cell line) and detected the change in the gene and protein expression levels of iNOS and VEGF by qRT‐PCR and W estern blotting. A transwell invasiveness assay was used to examine the changes in invasive ability of ACC ‐M cells. Cell growth was determined using a CCK ‐8 assay. Apoptosis and cell‐cycle phases were detected by flow cytometry. We found that silencing iNOS down‐regulated the expression of VEGF and then inhibited cell growth and invasiveness of SACC cells, while it increased apoptosis. Therefore, we concluded that iNOS can regulate VEGF expression and iNOS may be a therapeutic target.