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Immunohistochemical expression of matrix metalloprotease‐2 and matrix metalloprotease‐9 in the disks of patients with temporomandibular joint dysfunction
Author(s) -
Almeida Luis Eduardo,
Caporal Karina,
Ambros Viviane,
Azevedo Marina,
Noronha Lucia,
Leonardi Rosy,
Trevilatto Paula C
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12213
Subject(s) - temporomandibular joint , matrix metalloproteinase , immunostaining , immunohistochemistry , extracellular matrix , medicine , pathology , matrix metalloproteinase 3 , matrix (chemical analysis) , metalloproteinase , reduction (mathematics) , zymography , anatomy , chemistry , biochemistry , chromatography , geometry , mathematics
Purpose Matrix metalloproteases ( MMP s) are tissue‐remodeling enzymes that function during the remodeling process, such as in immune‐inflammatory diseases. Metalloprotease‐2 ( MMP ‐2) and metalloprotease‐9 ( MMP ‐9) are gelatinases that degrade several types of extracellular matrix collagen. It is hypothesized that in temporomandibular joint ( TMJ ) dysfunction, MMP ‐2 and MMP ‐9 expression levels may be elevated. Therefore, the objective of this study is to determine the association of MMP ‐2 and MMP ‐9 expression with temporomandibular joint dysfunction using an immunohistochemical approach to evaluate the joint disk. Material and Methods A total of 45 human temporomandibular joint samples were collected, with 36 samples in the test group (patients with anterior disk displacement with reduction ( n = 29) and without reduction ( n = 7)) and nine samples in the control group. The immunostaining of the TMJ disks was statistically compared between the groups ( P < 0.05). Results There was a statistically significant difference for the area of MMP ‐2 immunostaining between the control group and the displacement disks with reduction group ( ADD wR) ( P = 0.048) and between the groups with disk displacement and without reduction ( ADD woR) ( P = 0.029). The expression of MMP ‐2 was significantly elevated in the ADD woR group. Conclusion No statistically significant difference was found between the variable area of MMP ‐9 expression in the disk with and without disk displacement, as determined by immunohistochemical analysis. However, there was an elevation of MMP ‐2 expression in the disks of patients with displacement and without reduction (more severe alteration).