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Anti‐inflammatory effect of 635 nm irradiations on in vitro direct/indirect irradiation model
Author(s) -
Lim WonBong,
Choi Hongran,
Kim Jisun,
Kim Sangwoo,
Jeon SangMi,
Zheng Hui,
Kim DoMan,
Ko Youngjong,
Kim Donghwi,
Sohn HongMoon,
Kim OkJoon
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12204
Subject(s) - western blot , irradiation , chemistry , cytokine , inflammation , immunology , andrology , medicine , microbiology and biotechnology , endocrinology , biology , biochemistry , physics , nuclear physics , gene
Low‐level laser therapy (LLLT) has been promoted for its beneficial effects on tissue healing and pain relief. As during laser treatment it is possible to irradiate only a small area of the surface body or wound and, correspondingly, of a very small volume of the circulating blood, it is necessary to explain how its photomodification can lead to a wide spectrum of therapeutic effects. To establish the experimental model for indirect irradiation, irradiation with 635 nm was performed on immortalized human gingival fibroblasts (IGFs) in the presence of Porphyromonas gingivalis lipopolysaccharides (LPS). The irradiated medium was transferred to non‐irradiated IGFs which were compared with direct irradiated IGFs. The protein expressions were assessed by Western blot, and prostaglandin E 2 (PGE 2 ) was measured using an enzyme‐linked immunoassay. Reactive oxygen species (ROS) were measured by DCF‐DA; cytokine profiles were assessed using a human inflammation antibody array. Cyclooxygenase‐2 (COX‐2) protein expression and PGE 2 production were significantly increased in the LPS‐treated group and decreased in both direct and indirect irradiated IGFs. Unlike direct irradiated IGFs, ROS level in indirect irradiated IGFs was decreased by time‐dependent manners. There were significant differences of released granulocyte colony‐stimulating factor (G‐CSF), regulated on activated normal T‐cell expressed and secreted (RANTES), and I‐TAC level observed compared with direct and indirect irradiated IGFs. In addition, in the indirect irradiation group, phosphorylations of C‐Raf and Erk1/2 increased significantly compared with the direct irradiation group. Thus, we suggest that not only direct exposure with 635 nm light, but also indirect exposure with 635 nm light can inhibit activation of pro‐inflammatory mediators and may be clinically useful as an anti‐inflammatory tool.