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Histomorphometry in antigen‐induced arthritis of the rabbit temporomandibular joint
Author(s) -
Rafayelyan S.,
Radlanski R. J.,
Minden K.,
Pischon N.,
JostBrinkmann P.G.,
Präger T. M.
Publication year - 2015
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12201
Subject(s) - medicine , arthritis , temporomandibular joint , etanercept , h&e stain , pathology , cartilage , rheumatoid arthritis , surgery , immunohistochemistry , anatomy
Background Juvenile idiopathic arthritis ( JIA ) of the temporomandibular joint ( TMJ ) can cause severe growth disturbances of the craniomandibular system. Antigen‐induced arthritis ( AIA ) of the rabbit TMJ is simulating the inflammatory process of the TMJ in JIA . The aim of this study was to investigate the effect of a systemic administration of the tumor necrosis factor‐alpha ( TNF ‐α) antagonist etanercept on AIA in rabbits by means of three different histological staining methods. Methods After sensitization, a bilateral arthritis of the TMJ was induced and maintained by repeated intra‐articular administrations of ovalbumin in 12 N ew Z ealand white rabbits aged 10 weeks. From the 13th week of age, 6 of the 12 rabbits received weekly subcutaneous injections of etanercept, and the other 6 animals remained without therapy. Another 6 animals served as controls, receiving no treatment or intra‐articular injections at all. After euthanasia at the age of 22 weeks, all TMJ s were retrieved en bloc. Sagittal sections were cut and stained with hematoxylin–eosin ( H ‐ E ), S afranin‐ O for the evaluation of the M ankin score, and tartrate‐resistant acid phosphatase ( TRAP ). Results In the arthritis group, a chronic inflammation with degeneration of the articular cartilage was visible. In the etanercept group, the signs of cartilage degeneration were significantly reduced but present. In contrast, the joints in the control group were inconspicuous. A strong correlation between the M ankin score and TRAP ‐positive cells could be found. Conclusions Antigen‐induced arthritis causes severe damage in the TMJ of young rabbits. An improvement seems to be achievable by a systemic administration of etanercept.