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Detection of C andida albicans ADH1 and ADH2 m RNA s in human archival oral biopsy samples
Author(s) -
Bakri M. M.,
Can R. D.,
Holmes A. R.,
Rich A. M.
Publication year - 2014
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12193
Subject(s) - candida albicans , corpus albicans , leukoplakia , oral mucosa , staining , pathology , biology , biopsy , immunohistochemistry , oral and maxillofacial pathology , microbiology and biotechnology , medicine , cancer , genetics , orthodontics
Objectives The aim of this study was to investigate the relationship between expression of C andida albicans alcohol dehydrogenases ( ADH ) genes in archival formalin‐fixed paraffin‐embedded ( FFPE ) samples from biopsies of leukoplakia. Materials and Methods Archival FFPE samples were obtained from four sample groups: normal oral mucosa, non‐dysplastic leukoplakia, chronic hyperplastic candidosis ( CHC ), and non‐ CHC dysplastic leukoplakia. The presence of C. albicans was determined by periodic acid Schiff staining and by immunocytochemistry. C. albicans ADH1 and ADH2 m RNA s were detected using reverse transcription PCR . Results Candida albicans was detected in FFPE samples diagnosed as CHC (the histological diagnoses had been made by specialist oral pathologists, using uniform criteria), but not in any other sample group, including the non‐dysplastic leukoplakias. RT ‐ PCR confirmed a significant correlation between the expression of CaADH1 m RNA ( P = 0.000), but not for CaADH2 m RNA ( P = 0.056) in archival FFPE samples ( n = 31) from biopsies of leukoplakia. Conclusions Candida albicans was the predominant species in the lesions diagnosed as CHC , and the presence of C. albicans in CHC lesions was associated with a high expression of C. albicans ADH1 m RNA . There was no association between the presence of Candida and malignant transformation in the cases examined; however, the number of cases was limited and further studies are needed to further elucidate the role of C. albicans ADH1 in the pathogenesis of oral squamous cell carcinoma.