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miR‐146a and miR‐155 expression in PBMC s from patients with Sjögren's syndrome
Author(s) -
Shi Huan,
Zheng Lingyan,
Zhang Ping,
Yu Chuangqi
Publication year - 2014
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12187
Subject(s) - peripheral blood mononuclear cell , pathogenesis , medicine , microrna , rna , visual analogue scale , dry mouth , immunology , gene expression , gastroenterology , endocrinology , pathology , saliva , gene , biology , surgery , in vitro , biochemistry
Background An increasing number of studies have revealed that micro RNA (mi RNA ) contributes to the pathogenesis of autoimmune diseases. The objective of this study is to investigate the miR‐146a and miR‐155 levels in peripheral mononuclear blood cells from patients with primary Sjögren's syndrome ( pSS ) who were not receiving medications and to examine the correlations between these mi RNA levels and the clinical features of the disease. Method Using real‐time polymerase chain reaction analysis of mi RNA s, the miR‐146a and miR‐155 expression levels were assessed in peripheral mononuclear blood cells from 27 patients with pSS and 22 healthy controls, and the relationships between these mi RNA levels and the visual analog scale ( VAS ) scores for dry mouth, dry eyes, and parotid gland swelling were investigated. Results Compared with the healthy controls, the miR‐146a expression level was significantly increased in the patients with pSS ( P = 0.0182) and was positively correlated with the VAS scores for parotid swelling ( r = 0.4475, P = 0.0192) and dry eyes ( r = 0.4051, P = 0.0361). Although the miR‐155 expression level was significantly decreased in the patients with pSS ( P = 0.0131), the miR‐155 expression positively correlated with the VAS score for dry eyes ( r = 0.4894, P = 0.0096). Conclusion Our results demonstrated miR‐146a overexpression and miR‐155 underexpression in the peripheral mononuclear blood cells of the patients with pSS . Furthermore, the expression levels of these miRNAs correlated with the patients' clinical features. Our data suggest that miR‐146a and miR‐155 might play important roles in the pathogenesis of pSS and that their expression levels may be useful for diagnosing pSS and for predicting disease activity and therapeutic responses.