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Relationship between the epithelial expression of h MLH 1, MDM 2, and p63 and lower lip carcinogenesis
Author(s) -
Oliveira Denise Hélen Imaculada Pereira,
Sousa Lopes Maria Luiza Diniz,
Santana Sarmento Dmitry José,
Queiroz Lélia Maria Guedes,
Costa Miguel Márcia Cristina,
Silveira Éricka Janine Dantas
Publication year - 2014
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12144
Subject(s) - carcinogenesis , immunostaining , immunohistochemistry , immunoperoxidase , dysplasia , pathology , epithelial dysplasia , lower lip , carcinoma , biology , medicine , immunology , cancer , antibody , monoclonal antibody , surgery
Objectives To evaluate the relationship between the epithelial expression of h MLH 1, MDM 2, and p63 in lower lip carcinogenesis, comparing the immunostaining of these proteins in cases of actinic cheilitis ( AC ) and lower lip squamous cell carcinoma ( SCC ). Study Design Forty cases of AC and 40 cases of SCC were studied, both lesions were of lower lip. Histological sections of 3 μm were submitted to immunoperoxidase method, and 1000 cells were counted for immunohistochemical analysis of lesions. The results were analyzed quantitatively, and expression was compared by the M ann– W hitney, S tudent t ‐test, or one‐way ANOVA , adopting a level of significance of 5%. Results A higher percentage of epithelial cells expressing h MLH 1 was observed in cases of AC without dysplasia or mild dysplasia (721.23 ± 88.116), whereas fewer positive cells were observed in lower lip SSC s (255.03 ± 199.47) when compared to the AC group ( P < 0.001). Immunoexpression of MDM 2 was higher in SCC s of the lower lip compared with AC ( P = 0.019). For p63 protein, the expression was higher in AC than in SCC ( P = 0.045). Conclusion The present results showed changes in the immunoexpression of h MLH 1, MDM 2, and p63 in epithelial cells from premalignant and malignant lip disease, supporting the hypothesis that these alterations are related to the process of lower lip carcinogenesis.