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STAG 2 loss of expression is rare in aneuploid malignant salivary gland neoplasms
Author(s) -
Gomes Carolina Cavaliéri,
Bernardes Vanessa Fátima,
Odell Edward William,
Gomez Ricardo Santiago
Publication year - 2014
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12127
Subject(s) - aneuploidy , biology , immunohistochemistry , salivary gland , ploidy , chromosome instability , pathology , centromere , flow cytometry , cancer research , microbiology and biotechnology , chromosome , immunology , gene , medicine , genetics , biochemistry
Background STAG 2 depletion leads to loss of centromere cohesion in vitro , and some human neoplasms have been shown to lose expression of this protein. As a result, STAG 2 loss has been shown to cause chromosomal instability and aneuploidy in human cancer cell lines. Methods We tested the hypothesis that aneuploid salivary gland tumours lose immunoexpression of STAG 2 compared with diploid tumours using image cytometry to determine DNA ploidy and immunohistochemistry to assess STAG 2 protein expression in 30 malignant salivary gland neoplasms. Results There was no difference in the immunoexpression of STAG 2 between aneuploid ( n = 9) and diploid ( n = 21) samples. In all but two samples, more than 50% of cells stained for STAG 2. Conclusion Aneuploidy in human salivary gland carcinomas is not driven by loss of expression of STAG 2.