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Metallothionein immunoexpression in selected benign epithelial odontogenic tumors
Author(s) -
Johann Aline Cristina Batista Rodrigues,
Caldeira Patrícia Carlos,
Souto Giovanna Ribeiro,
Abreu Mauro Henrique Nogueira Guimarães,
Aguiar Maria Cássia Ferreira,
Mesquita Ricardo Alves
Publication year - 2014
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12122
Subject(s) - immunohistochemistry , metallothionein , pathology , odontogenic , proliferating cell nuclear antigen , ameloblastoma , staining , adenomatoid odontogenic tumor , ki 67 , biology , medicine , anatomy , maxilla , biochemistry , gene
Background Odontogenic tumors exhibited variable biologica behaviors. Metallothionein ( MT ) is correlated with the cellular homeostasis of essential metals, cellular differentiation, and proliferation. The core goals of this study are (i) to report and to compare MT expression among benign epithelial odontogenic tumors; (ii) to correlate MT with cellular proliferation index; and (iii) to evaluate the influence of the inflammatory infiltrate on MT expression. Materials and Methods Ten cases of solid ameloblastomas ( SAB s), 4 squamous odontogenic tumors ( SOT s), 5 adenomatoid odontogenic tumors ( AOT s), and 3 calcifying epithelial odontogenic tumors ( CEOT s) were subjected to immunohistochemical to anti‐ MT , anti‐Ki‐67, and anti‐ PCNA . Statistical analysis was performed using BioEstat ® 4.0. Results Metallothionein staining was found to be the highest in the SAB s (93.1%), followed by SOT s (52.9%), AOT s (38.4%), and CEOT s (0%). MT staining exhibited statistically significant differences between the SAB s and the SOT s ( P = 0.0047) and the AOT s ( P = 0.0022). A weak‐to‐strong positive correlation between IMT and IK or IP was observed in SAB s and SOT s, whereas a strong negative correlation was observed in AOT s. No differences in IMT , IK , and IP were observed between inflammation groups A and B. Conclusions The increased MT expression observed in the SAB s might be correlated with clinical behavior (local invasiveness and high rate of recurrence). In the SAB s and SOT s, MT plays a role in the stimulation of cellular proliferation. In contrast, MT can inhibit cellular proliferation in the AOT . The IMT , IK , and IP are not affected by inflammation.