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Expression of molecules related to AKT pathway as putative regulators of ameloblastoma local invasiveness
Author(s) -
Cecim Rodolpho L.,
Carmo Hicso A. F.,
Kataoka Maria S. S.,
Freitas Vanessa M.,
Melo Alves Júnior Sérgio,
Pedreira Erick N.,
Jaeger Ruy G.,
Pinheiro Joao J. V.
Publication year - 2014
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12103
Subject(s) - ameloblastoma , protein kinase b , pi3k/akt/mtor pathway , pathology , immunohistochemistry , cancer research , stromal cell , cyclin d1 , stroma , medicine , biology , signal transduction , cancer , cell cycle , molar , microbiology and biotechnology , dentistry
Background Ameloblastoma is an odontogenic neoplasm with local invasiveness and high recurrence. We previously suggested that growth factors, matrix metalloproteinases ( MMP s), and TIMP s influence ameloblastoma invasiveness ( Pathol. Res. Pract., 208, 2012, 225; Oral. Surg. Oral. Med. Oral. Pathol. Oral Radiol. Endod., 111, 2011, 474). Signals generated by this molecular network would be transduced by ERK 1/2 pathway ( Oral. Surg. Oral. Med. Oral. Pathol. Oral Radiol. Endod., 111, 2011, 474). Others signaling pathways may influence ameloblastoma biology. Here, we studied expression of AKT and related molecules in ameloblastoma. Methods Fourteen cases of solid/multicystic ameloblastomas were examined. Immunohistochemistry was carried out to detected AKT (phospho‐ AKT ), NF ‐қ B (phospho‐ NF ‐қB), β‐catenin, cyclin‐ D 1, and COX ‐2 in ameloblastoma samples. These molecules were evaluated in neoplastic cells and stroma. Results All proteins were detected in ameloblastoma. Expression of these markers was quantified and compared. Spearman's rank test was carried out to address positive correlations between proteins ( P < 0.05). Ameloblastoma had a significant positive correlation of AKT (phospho‐ AKT ) with β‐catenin. β‐catenin correlated with Cyclin‐D1 and COX ‐2 in neoplastic cells. AKT (phospho‐ AKT ) correlated with β‐catenin; β‐catenin with C yclin‐ D 1; AKT (phospho‐ AKT ) with NF ‐қB (phospho‐ NF ‐қB); and NF ‐қB (phospho‐ NF ‐қB) with COX ‐2 in stromal cells. Conclusions Results suggest that proteins studied are present and probably involved in a functional pathway in neoplastic cells and stroma and may therefore influence the local invasiveness of ameloblastoma.