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Immunohistochemical detection of H elicobacter pylori without association of TLR 5 expression in oral squamous cell carcinoma
Author(s) -
Grimm Martin,
Munz Adelheid,
Exarchou Alexandros,
Polligkeit Joachim,
Reinert Siegmar
Publication year - 2014
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12082
Subject(s) - tlr5 , immunohistochemistry , cancer , western blot , helicobacter pylori , blot , biology , cancer research , immunology , medicine , pathology , immune system , toll like receptor , innate immune system , gene , biochemistry
Background Approximately 15% of human deaths from cancer are associated with chronic viral or bacterial infections. Helicobacter pylori ( HP ), a flagellated, Gram‐negative, spiral, microaerophilic bacteria is considered to be the most common chronic bacterial infection in humans. Toll‐like receptor 5 ( TLR 5) is involved in recognition of bacterial flagella and is thought to promote tumour growth through inflammation‐dependent mechanisms in epithelial cells. Methods Expression of HP and TLR 5 was analysed in OSCC specimen ( n = 191) by immunohistochemistry. TLR 5 expression specificity was conducted by Western blotting in cancer cell lines ( BICR 3, BICR 56). TLR 5‐stained sections were scanned and digitally analysed using ImageJ and the immunomembrane plug‐in. HP expression and TLR 5 expression were associated with clinicopathological characteristics and impact on survival. Results Helicobacter pylori detection was significantly associated with recurrence of the tumour, whereas TLR 5 expression was not. Multivariate analysis demonstrated HP expression as an independent prognostic factor ( P = 0.0260). TLR 5 specificity was confirmed by W estern blot analysis. Conclusions For the first time, this study provides evidence that immunohistochemically detected HP expression in OSCC is associated with reduced disease‐free survival in a large patient cohort. Although TLR 5 was not associated with any clinicopathological characteristics or impact on survival, investigation of the TLR family seems to be reasonable due to the possible existence of other pathogenic bacterial or viral compounds in oral cavity cancer.