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Detection of claudin‐4 in salivary gland neoplasms (a study utilizing RT ‐ PCR and immunohistochemistry)
Author(s) -
Abd ElGhani Safa F.,
Kasem Rehab F.,
Ghallab Noha A.,
Shaker Olfat G.
Publication year - 2013
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12070
Subject(s) - claudin , salivary gland , adenoid cystic carcinoma , immunohistochemistry , mucoepidermoid carcinoma , pathology , pleomorphic adenoma , salivary gland cancer , biology , carcinoma , metastasis , medicine , cancer , tight junction , microbiology and biotechnology
Background Claudins are transmembrane proteins of tight junctions emerging as targets for diagnosis, prediction of prognosis, disease recurrence, and metastasis. Our goal was to evaluate expression of claudin‐4 in the most common benign and malignant salivary gland neoplasms. Methods Claudin‐4 gene levels and protein expression were determined by real‐time polymerase chain reaction ( PCR ), and immunohistochemistry in a total of 30 specimens containing normal salivary tissue, pleomorphic adenoma, W arthin's tumor, mucoepidermoid carcinoma, and adenoid cystic carcinoma. Results We identified down‐regulation of claudin‐4 gene levels and protein expression from normal control to benign salivary gland neoplasms and reached their lowest values in the malignant salivary gland neoplasms. Conclusions Low claudin‐4 expression could be considered as a sign of increasing cellular disorientation and invasion of salivary gland tumors.