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Reduced immune responses to purified protein derivative and C andida albicans in oral lichen planus
Author(s) -
SimarkMattsson Charlotte,
Eklund Christina
Publication year - 2013
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12069
Subject(s) - oral lichen planus , peripheral blood mononuclear cell , immune system , immunology , candida albicans , antigen , tumor necrosis factor alpha , pathogenesis , cytokine , interleukin , cellular immunity , biology , medicine , microbiology and biotechnology , in vitro , biochemistry
Background Impairment of cellular immunity is reported in lichen planus, an autoimmune disease affecting mucosae and skin. Our aim was to investigate immune responses directed against a set of microbial antigens in patients with oral lichen planus and in matched controls. Methods Venous blood was obtained, and the mononuclear cells were enriched by density gradient centrifugation. The proliferation of peripheral blood mononuclear cells was assessed, following stimulation with purified protein derivative ( PPD ), Candida albicans , phytohemagglutinin or when cells were left unstimulated, after three or six days of cell culture. The production of interleukin‐1ß ( IL ‐1ß), IL ‐2, IL ‐4, IL ‐5, IL ‐6, IL ‐10, IL ‐12, IL ‐13, IL ‐17, interferon‐γ ( IFN ‐γ), tumour necrosis factor‐α ( TNF ‐α), G‐ CSF , GM ‐ CSF , MCP ‐1, MIP ‐ß was assessed in supernatants using the Bio‐plex ® assay and was complemented with ELISA for selected cytokines. Results Patients with oral lichen planus demonstrated reduced proliferative responses against PPD ( P < 0.05) and C . albicans ( P < 0.05). The majority of investigated cytokines, including the pro‐inflammatory, IFN ‐γ and TNF ‐α were expressed at reduced levels in PPD ‐stimulated supernatants from patients with oral lichen planus. Conclusions Collectively, the findings suggested that memory lymphocytes from patients with oral lichen planus ( OLP ) may have an impaired functional ability to react against certain recall antigens, as part of a generalized response, which may reflect immune regulatory processes. Further studies are needed to clarify the mechanisms of down‐regulation in OLP pathogenesis and progression.