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Inhibition of autophagy enhances cisplatin cytotoxicity in human adenoid cystic carcinoma cells of salivary glands
Author(s) -
Ma Ben,
Liang Lizhong,
Liao Guiqing,
Liang Yujie,
Liu Haichao,
Zheng Guangsen,
Su Yuxiong
Publication year - 2013
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12066
Subject(s) - autophagy , salivary gland , cisplatin , cytotoxicity , chemotherapy , adenoid cystic carcinoma , cancer research , biology , mtt assay , western blot , cell culture , programmed cell death , pathology , medicine , apoptosis , carcinoma , in vitro , biochemistry , gene , genetics
Background The relationship between autophagy and chemotherapy in cancer has been studied a lot recent years. However, there is currently no study on the role of autophagy in chemotherapy of adenoid cystic carcinoma ( ACC ) of human salivary glands. We hypothesized that autophagy plays a protective role for human salivary gland ACC cells during chemotherapy, diminishes the effect of treatment, and ultimately results in poor sensitivity to chemotherapy. Materials and Methods After inhibition of autophagy by 5 mM 3‐methyladenine (3 MA ), 20 μM C hloroquine ( CQ ), or B eclin‐1 sh RNA , we examined the sensitivity of human salivary gland ACC cells to different concentrations of cis‐diamminedichloroplatinum ( CDDP ) using MTT assay. Also, levels of autophagy in ACC cells treated by CDDP were assessed by western blot, GFP ‐ LC 3 fluorescence and transmission electron microscopy ( TEM ). Results Inhibition of autophagy induced by 3 MA , CQ , or B eclin‐1 sh RNA could all enhance human salivary gland ACC cell death treated by CDDP . And, levels of autophagy in these cells showed a significant increase after treated by CDDP . Conclusion Autophagy played a protective role for human salivary gland ACC cells during CDDP chemotherapy. Inhibition of autophagy in these cells could enhance cisplatin cytotoxicity‐effects. These findings indicate a novel and promising way to reduce chemotherapy resistance and improve treatment outcome in human salivary gland ACC .