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Macrophage migration inhibitory factor and oral cancer
Author(s) -
França Cristiane M.,
Batista Aline C.,
Borra Ricardo C.,
VentiadesFlores Jose A.,
Mendonça Elismauro F.,
Deana Alessandro M.,
MesquitaFerrari Raquel A.,
Natali Caly Decio,
Mello Rode Sigmar,
Faria Miriam R.
Publication year - 2013
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12011
Subject(s) - macrophage migration inhibitory factor , stromal cell , cancer , pathology , medicine , immunohistochemistry , stroma , carcinogenesis , parenchyma , cytokine , macrophage , cancer research , immunology , biology , in vitro , biochemistry
Background Macrophage migration inhibitory factor ( MIF ) is a pleiotropic cytokine with pro‐inflammatory functions and involved in tumorigenesis. The aim of this study was to evaluate the expression and localization of the macrophage MIF in oral squamous carcinoma ( OSC ). In addition, the relationship between MIF expression and clinicopathological parameters such as survival data, tobacco use, alcohol habits, TNM stage, tumor graduation, and peritumoral inflammatory infiltrate were evaluated. Methods Using immunohistochemistry, expression and localization of MIF was detected in 44 specimens of OSC . The absolute number and relative proportions of MIF ‐positive cells detected were also determined separately for tumor parenchyma vs. stroma. All counts were determined from 10 consecutive high‐power fields using an integration graticule. Moreover, some parameters were analyzed separately for lip and intra‐oral cancers. Results Migration inhibitory factor‐positive cells were observed in both the tumor parenchyma and in inflammatory cells of all specimens. In contrast, MIF expression was not detected in tumoral nests associated with poorly differentiated tumors. In specimens of lip cancer, a greater number of MIF ‐positive stromal immune cells were detected than in intra‐oral cancer specimens ( M ann– W hitney test, P = 0.049). Conclusions Oral squamous carcinoma cells consistently express MIF independent of their location. Lip tumors presented more MIF ‐positive peritumoral inflammatory cells, similar to control, suggesting that immunological differences in leukocyte activation exist between in lip and intra‐oral cancers.