Premium
Colonization of the oral cavity by yeasts in patients with chronic renal failure undergoing hemodialysis
Author(s) -
Godoy Janine S. R.,
Souza BonfimMendonça Patrícia,
Nakamura Sandra S.,
Yamada Sérgio S.,
ShinobuMesquita Cristiane,
Pieralisi Neli,
Fiorini Adriana,
Svidzinski Terezinha I. E.
Publication year - 2013
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1111/jop.12003
Subject(s) - caspofungin , fluconazole , voriconazole , amphotericin b , itraconazole , nystatin , medicine , hemodialysis , corpus albicans , candida albicans , microbiology and biotechnology , minimum inhibitory concentration , dialysis , biology , gastroenterology , antifungal , antibiotics
Objectives To determine the frequency of yeast in the oral cavity of patients with chronic renal failure, undergoing hemodialysis ( PCRFH ); identification and antifungal susceptibility profile of yeast and demographic profile of patients. Methods We performed mouthwash in 146 PCRFH ; the rinse fluid was collected and cultured, yeasts grown were identified by phenotypic and molecular methods. The antifungal susceptibility profile was determined against nystatin, amphotericin B, fluconazole, voriconazole, and caspofungin based in Clinical and Laboratory Standards Institute (document M 27‐ A 3). Results Positive culture was observed in 39% of patients, of whom 53% were women; the median of dialysis time was 2.9 years. The age of the colonized patients varied between 26 and 84 years, with a median of 52.5 years. PCRFH over 45 years were significantly more colonized ( P = 0.0108) as well as denture wearers (84.0%). We isolated 81 yeasts, predominantly C andida albicans (63%) followed by C andida glabrata . In general, yeasts were sensitive to the evaluated antifungal agents, but there was significant variation in the minimum inhibitory concentration, especially among non‐ C . albicans Candida ( NCAC ) compared to fluconazole, caspofungin, and amphotericin B. NCAC required significantly higher concentrations of fluconazole ( P < 0.01). Conclusion The rate of colonization by yeasts in PCRFH was high, and there was variability in species distribution and antifungal susceptibility profile. These results are little known in this group of patients and are important for controlling the risk of developing invasive fungal infections.