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Regeneration characteristics of different dental derived stem cell sheets
Author(s) -
Hu Lei,
Zhao Bin,
Gao Zhenhua,
Xu Junji,
Fan Zhipeng,
Zhang Chunmei,
Wang Jinsong,
Wang Songlin
Publication year - 2020
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/joor.12839
Subject(s) - periodontal ligament stem cells , stem cell , regeneration (biology) , dental pulp stem cells , microbiology and biotechnology , mesenchymal stem cell , chemistry , materials science , biology , alkaline phosphatase , biochemistry , enzyme
Background Although cell sheets have gained much interest as a non‐scaffold strategy for tissue regeneration, the regenerative features of different cell sheets remain unclear. Objective In this study, we aimed to compare the regeneration characteristics of cell sheets derived from dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs) and stem cells of the apical papilla (SCAPs). Methods Dental pulp stem cells, PDLSCs and SCAPs from the same individual were acquired and induced to form sheets using 20 μg/mL vitamin C. Immunofluorescence staining was used to detect the expression of collagen I, fibronectin, integrin β1 and vimentin. Real‐time PCR was used to determine NANOG , OCT4 , SOX2 and TERT gene expression. The cell sheets with hydroxyapatite/tricalcium phosphate were transplanted into nude mice subcutaneously to evaluate tissue regeneration characteristics. Results No obvious differences were found in the histological structure and extracellular matrix protein expression between DPSC, PDLSC and SCAP sheets. Dental pulp stem cell sheet showed higher expression of OCT4 and TERT than PDLSC and SCAP sheets. All three cell sheets displayed the ability of mineral tissue formation and highly expressed periostin. The tissue derived from DPSC sheet showed higher CD31 expression and porous fibres compared with that from the others. The tissue fibres formed from PDLSC sheet were directionally arranged, while the tissue derived from SCAP sheet showed highest mineral tissue formation. Conclusion Although in vitro DPSC, PDLSC and SCAP cell sheets have similar characteristics, their regenerative characteristics in vivo are different, with each showing potential application for regeneration of different tissues.