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Transcranial direct‐current stimulation reduces nociceptive behaviour in an orofacial pain model
Author(s) -
Scarabelot Vanessa L.,
Oliveira Carla,
Medeiros Liciane F.,
Macedo Isabel C.,
Cioato Stefania G.,
Adachi Lauren Naomi S.,
Paz Ana Helena,
Souza Andressa,
Caumo Wolnei,
Torres Iraci L. S.
Publication year - 2019
Publication title -
journal of oral rehabilitation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 93
eISSN - 1365-2842
pISSN - 0305-182X
DOI - 10.1111/joor.12726
Subject(s) - orofacial pain , nociception , transcranial direct current stimulation , neuroscience , stimulation , medicine , psychology , physical medicine and rehabilitation , anesthesia , physical therapy , receptor
Summary Background Transcranial direct‐current stimulation ( tDCS ) is a noninvasive method of brain stimulation suggested as a therapeutic tool for pain and is related to the reversal of maladaptive plasticity associated with chronic pain. Objectives This study investigated the effect of tDCS , a non‐pharmacological therapy, on local mechanical hyperalgesia, and remote thermal hyperalgesia in rats submitted to orofacial inflammatory pain model, by facial von Frey and hot plate tests, respectively. In addition, we evaluated levels of BDNF , NGF , IL ‐10 and IL ‐6 in the brainstem and blood serum of these animals at 24 hours and 7 days after the end of tDCS treatment. Methods Rats were subjected to temporomandibular joint pain and treated with tDCS . The animals were divided into control, pain and pain + treatment groups. Mechanical and thermal hyperalgesia were evaluated at baseline, 7 days after administration of complete Freund's adjuvant, and immediately, 24 hours, and 7 days after the tDCS treatment. Neuroimmunomodulators levels were determined by ELISA . Statistical analyses were performed by ( GEE )/Bonferroni (behavioural tests), three‐way ANOVA / SNK (neurochemical tests) and Kruskal‐Wallis (histological analysis). Results Transcranial direct‐current stimulation reduced mechanical and thermal hyperalgesia ( P < 0.01). We observed interaction between factors (pain and treatment) increasing brainstem BDNF ( P < 0.01) and NGF ( P < 0.05) levels. Furthermore, we found an increase in IL ‐6 and IL ‐10 levels in the brainstem at 24 hours and 7 days after tDCS , respectively. Conclusion We showed that tDCS reduces thermal and mechanical hyperalgesia induced by orofacial pain until 7 days after treatment. These findings demonstrate that tDCS was effective in the control of orofacial inflammatory pain.