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Targeted proteomic analysis of habitual coffee consumption
Author(s) -
Cornelis M. C.,
Gustafsson S.,
Ärnlöv J.,
Elmståhl S.,
Söderberg S.,
Sundström J.,
Michaëlsson K.,
Lind L.,
Ingelsson E.
Publication year - 2018
Publication title -
journal of internal medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.625
H-Index - 160
eISSN - 1365-2796
pISSN - 0954-6820
DOI - 10.1111/joim.12703
Subject(s) - medicine , prospective cohort study , epidemiology , tumor necrosis factor alpha , body mass index , leptin , disease , endocrinology , obesity
Background Coffee drinking has been implicated in mortality and a variety of diseases but potential mechanisms underlying these associations are unclear. Large‐scale systems epidemiological approaches may offer novel insights to mechanisms underlying associations of coffee with health. Objective We performed an analysis of known and novel protein markers linked to cardiovascular disease and their association with habitual coffee intake in the Prospective Study of the Vasculature in Uppsala Seniors ( PIVUS , n = 816) and followed up top proteins in the Uppsala Longitudinal Study of Adult Men ( ULSAM , n = 635) and EpiHealth ( n = 2418). Methods In PIVUS and ULSAM , coffee intake was measured by 7‐day dietary records whilst a computer‐based food frequency questionnaire was used in EpiHealth. Levels of up to 80 proteins were assessed in plasma by a proximity extension assay. Results Four protein–coffee associations adjusted for age, sex, smoking and BMI , met statistical significance in PIVUS ( FDR < 5%, P < 2.31 × 10 −3 ): leptin ( LEP ), chitinase‐3‐like protein 1 ( CHI 3L), tumour necrosis factor ( TNF ) receptor 6 and TNF ‐related apoptosis‐inducing ligand. The inverse association between coffee intake and LEP replicated in ULSAM (β, −0.042 SD per cup of coffee, P = 0.028) and EpiHealth (β, −0.025 SD per time of coffee, P = 0.004). The negative coffee– CHI 3L association replicated in EpiHealth (β, −0.07, P = 1.15 × 10 −7 ), but not in ULSAM (β, −0.034, P = 0.16). Conclusions The current study supports an inverse association between coffee intake and plasma LEP and CHI 3L1 levels. The coffee– CHI 3L1 association is novel and warrants further investigation given links between CHI 3L1 and health conditions that are also potentially influenced by coffee.