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Does the use of microfluidic sperm sorting for the sperm selection improve in vitro fertilization success rates in male factor infertility?
Author(s) -
Ozcan Pinar,
Takmaz Taha,
Yazici Melis Gökce Kocer,
Alagoz Oya Akcin,
Yesiladali Mert,
Sevket Osman,
Ficicioglu Cem
Publication year - 2021
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.14539
Subject(s) - sperm , infertility , andrology , in vitro fertilisation , medicine , assisted reproductive technology , unexplained infertility , human fertilization , antral follicle , gynecology , pregnancy rate , male infertility , sperm washing , pregnancy , biology , insemination , ovary , genetics , anatomy
Aim To evaluate the clinical outcome of assisted reproductive technology (ART) cycles in couple with male infertility, where the spermatozoa were selected using either a conventional gradient‐density centrifugation technique or microfluidic sperm sorting. Methods A total of 181 patients who underwent in vitro fertilization (IVF) because of male factor infertility at the IVF center of Bezmialem and Yeditepe University Hospital were included in this study. All patients were divided into two groups according to the sperm selection method: group I ( n = 91): microfluidic sperm‐sorting chip; group II ( n = 90): density‐gradient centrifugation. Data collected included male and female age, type of infertility, duration of infertility, previous IVF attempts, smoking, antral follicle count, total dosage of gonadotropins, maximum estradiol levels, duration of stimulations, endometrial thickness on human chorionic gonadotropin day, total number of oocytes retrieved, number of mature oocytes retrieved, number of pronuclear (PN), sperm parameters, clinical PR and ongoing PR. Results There was no statistically significant difference in clinical PR and ongoing PR between groups (49.5% vs 40%, P = 0.2; 44% vs 36.7%, P = 0.3; respectively). The improvement in pregnancy rate was more prominent in patients where the female partner's age is higher than 35 ( P = 0.09) and men have a total motile sperm count between 1 and 5 million ( P < 0.01). Conclusion Microfluidic devices, “labs‐on‐a‐chip,” are a disposable, easy to use, and inexpensive method for sperm sorting. Our results show that IVF success rates might improve with the use of a microfluidic sperm‐sorting chip for sperm selection in male infertility.