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miR ‐483 is d ownregulated in pre‐eclampsia via targeting insulin‐like growth factor 1 ( IGF1 ) and regulates the PI3K / Akt / mTOR pathway of endothelial progenitor cells
Author(s) -
Han Li,
Luo Qingqing,
Peng Minggang,
Zhang Yang,
Zhu Xiaohui
Publication year - 2021
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.14412
Subject(s) - pi3k/akt/mtor pathway , progenitor cell , umbilical cord , medicine , protein kinase b , endothelial progenitor cell , western blot , eclampsia , vascular endothelial growth factor , andrology , stem cell , cancer research , immunology , microbiology and biotechnology , biology , pregnancy , signal transduction , vegf receptors , biochemistry , gene , genetics
Aim Pre‐eclampsia is a serious pregnancy‐specific disease with an incidence of 9.4%. MicroRNAs play a key role in regulating factors in pre‐eclampsia, but related research is still limited. This study aims to reveal the role and potential mechanisms of miR‐483 in pre‐eclampsia. Methods miR‐483 was detected in venous blood, umbilical cord blood and placental tissue of pre‐eclampsia patients by Real‐time Quantitative polymerase chain reaction (qRT‐PCR). Insulin‐like growth factor (IGF1) and miR‐483 were detected by qRT‐PCR and western blot in endothelial progenitor cells isolated from fetal umbilical cord blood. miR‐483 was overexpressed and inhibited to detect changes of IGF1 and PI3K/Akt/mTOR pathway in endothelial progenitor cells by qRT‐PCR and western blot. Results miR‐483 was downregulated in venous blood, umbilical cord blood and placental tissue of pre‐eclampsia patients. In endothelial progenitor cells, overexpression of miR‐483 inhibited the expression of IGF1, and inhibition of miR‐483 promoted the expression of IGF1. miR‐483 regulates the expression of PI3K, Akt, and mTOR in endothelial progenitor cells. Conclusion miR‐483 is downregulated in pre‐eclampsia and regulates endothelial progenitor cells by targeting IGF1. miR‐483 is a potential alternative for diagnosing and treating pre‐eclampsia.