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Searching for valuable differentially expressed miRNAs in postmenopausal osteoporosis by RNA sequencing
Author(s) -
Wang Randong,
Lu Aiping,
Liu Wangyan,
Yue Juan,
Sun Qiang,
Chen Jiao,
Luan Huijie,
Zhai Yaling,
Li Bing,
Jiang Zhongcai,
Li Yingnan
Publication year - 2020
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.14307
Subject(s) - microrna , kegg , rna , gene , messenger rna , postmenopausal osteoporosis , computational biology , osteoporosis , bioinformatics , biology , gene expression , medicine , genetics , gene ontology , endocrinology , bone mineral
Aim Postmenopausal osteoporosis is a systemic and chronic bone disease in women. In order to understand the pathological mechanism of postmenopausal osteoporosis, we aimed to find the potential differentially expressed miRNAs in the disease. Methods Firstly, RNA sequencing was used to identify differentially expressed miRNAs, followed by the construction of the miRNA–target mRNA regulatory network. Then, Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes were used to analyze the biological function of target mRNAs. Finally, electronic validation of identified differentially expressed miRNAs and target mRNAs was performed. Results A total of 33 differentially expressed miRNAs (18 upregulated and 15 downregulated miRNAs) and 6820 miRNA–mRNA pairs were identified. Among which, seven miRNAs with high degree including hsa‐miR‐17‐5p, hsa‐miR‐1‐3p, hsa‐miR‐193b‐3p, hsa‐miR‐125b‐5p, hsa‐miR‐10b‐5p, hsa‐miR‐100‐5p and hsa‐miR‐30a‐3p were obtained in the miRNA–mRNA regulatory network. TGF‐beta was the most significantly enriched signaling pathway of target mRNAs. The electronic validation result of hsa‐miR‐1‐3p, hsa‐miR‐193b‐3p, hsa‐miR‐10b‐5p, hsa‐miR‐100‐5p, hsa‐miR‐133b, hsa‐miR‐708‐5p, CRK, RAB5C, CCND1 and PCYOX1 was consisted with the RNA sequencing analysis. Conclusion Dysfunctional miRNAs may play significant roles in postmenopausal osteoporosis.