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Immunohistochemical expression of CD44v9 and 8‐OHdG in ovarian endometrioma and the benign endometriotic lesions adjacent to clear cell carcinoma
Author(s) -
Niiro Emiko,
Kawahara Naoki,
Yamada Yuki,
Yoshimoto Chiharu,
Shimada Keiji,
Sudo Tamotsu,
Kobayashi Hiroshi
Publication year - 2019
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.14093
Subject(s) - medicine , endometriosis , cd44 , immunohistochemistry , malignant transformation , clear cell carcinoma , pathology , clear cell , cell , carcinoma , biology , genetics
Aim Expression of CD44 variant isoforms (CD44v) promotes the synthesis of reduced glutathione and contributes to reactive oxygen species defense through up‐regulation of the intracellular antioxidant. The aim of the study was to investigate the expression of CD44v9 and oxidative DNA damage marker, 8‐OHdG, in benign ovarian endometrioma (OE) and OE harboring clear cell carcinomas (CCC). Methods A retrospective study was performed at the Department of Gynecology, Nara Medical University hospital from January 2006 to December 2012. Patients with histologically confirmed benign OE ( n = 27) and OE harboring areas of CCC ( n = 8) were selected. Tissue samples were immunohistochemically analyzed for the presence of CD44v9 and 8‐OHdG using avidin‐biotin complex method. Results CD44v9 was located on the cell membrane of endometriotic epithelial cells and expressed in 88.9% (24/27) of benign OE tissues. Only 25.0% (2/8) of benign endometriotic lesions adjacent to CCC was found to stain weakly for CD44v9. Percentage of CD44v9 positive cells was 68.5 ± 20.2% (mean ± standard deviation) of benign OE, 16.7 ± 16.5% of CCC endometriotic tissue ( P < 0.001). Compared to benign OE, CCC endometriotic tissue showed a significant increase in the proportion of 8‐OHdG expression (77.3 ± 22.5% vs 94.9 ± 3.0%, P = 0.049). A significant negative correlation was observed between CD44v9 status and 8‐OHdG nuclear expression ( r = −0.458, P = 0.006). Conclusion Alterations in CD44v9 and 8‐OHdG may be associated with malignant transformation of benign OE.