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Role of oxidative stress in follicular fluid on embryos of patients undergoing assisted reproductive technology treatment
Author(s) -
Terao Hiromi,
WadaHiraike Osamu,
Nagumo Aiko,
Kunitomi Chisato,
Azhary Jerilee M. K.,
Harada Miyuki,
Hirata Tetsuya,
Hirota Yasushi,
Koga Kaori,
Fujii Tomoyuki,
Osuga Yutaka
Publication year - 2019
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.14040
Subject(s) - follicular fluid , oxidative stress , andrology , human fertilization , intracytoplasmic sperm injection , embryo , assisted reproductive technology , oocyte , medicine , blastocyst , antioxidant , embryogenesis , reactive oxygen species , sperm , in vitro fertilisation , biology , infertility , microbiology and biotechnology , biochemistry , pregnancy , anatomy , genetics
Aim Oxidative stress (OS) is defined as an imbalance between oxidants and antioxidants in favor of the oxidants, and the disruption of redox signaling leads to molecular damages. This study was conducted to clarify the effect of OS in individual follicular fluid (FF) on oocyte fertilization and embryonic division. Methods A total of 124 patients who underwent assisted reproductive technology treatment in our hospital underwent intracytoplasmic sperm injection and 211 FF were collected. The ova were fertilized, and embryos were individually cultured until cleavage stage or blastocyst stage and classified according to the Veeck classification system or Gardner classification system. Results The follicular fluid corresponding to the ovum was analyzed by measuring the OS marker, the reactive oxygen metabolites (d‐ROM test), and the antioxidant marker (biological antioxidant potential marker [BAP] test), and the relation between these markers and clinical parameters was analyzed. The value of d‐ROM was correlated with the proper fertilization status and formation of good quality cleavage embryo, whereas the elevated value of BAP was observed in better embryonic development group. Oxidative stress index, defined as d‐ROM/BAP × 100 clearly indicated that lower oxidative stress index was associated with better fertilization status and embryo development. Conclusion Our results clearly indicate that the balance between OS and antioxidant capacity in FF at the time of oocyte retrieval is possibly important in the processes of fertilization and embryo division; thus, we propose that oxidative status and balance in FF might be used as novel biomarkers in assisted reproductive technology.