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Implications from early life stress on the development of mouse ovarian follicles: Focus on oxidative stress
Author(s) -
Ghatebi Mina,
Zavareh Saeed,
Lashkarbolouki Taghi,
Elahdadi Salmani Mahmoud
Publication year - 2019
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.14007
Subject(s) - malondialdehyde , oxidative stress , superoxide dismutase , glutathione peroxidase , catalase , andrology , medicine , antral follicle , endocrinology , glutathione , antioxidant , ovulation , biology , biochemistry , ovary , enzyme , hormone
Aim The early life stress has significant long‐term effects on the development of the offspring. This study was undertaken to verify if maternal separation as a stressor agent affects the oxidative status and developmental competence of mouse pre‐antral follicles (PF) during in vitro culture period. Methods Female litters of National Medical Research Institute mice were divided into two groups: maternally separated group (MS), separated from the mothers for 6 h per day from postnatal days 2–16; and the rest considered as the control group, which left undisturbed over the 14 days. The litters were sacrificed and the ovarian tissue was harvested to isolate the PF. The PF were in vitro cultured up to 12th day when ovulation was induced. The developmental parameters and oxidative status (i.e., total antioxidant capacity and Malondialdehyde levels, as well as the activities of superoxide dismutase, glutathione peroxidase and catalase) were assessed. Results The rates of survival, antrum formation, ovulation and oocyte maturation of PF derived from the MS group were significantly lower compared with those of the control group. Furthermore, the Malondialdehyde level of the MS group was significantly higher than that of the control group. By contrast, the total antioxidant capacity level was lower in the MS group with respect to the control group. Also, the activity of superoxide dismutase, glutathione peroxidase and catalase of PF, derived from the MS group, was significantly lower compared with those of the control group. Conclusion Early life stress damages the developmental competence of mouse PF through induction of oxidative stress.