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Bisphenol A promotes the proliferation of leiomyoma cells by GPR30‐EGFR signaling pathway
Author(s) -
Li Zemin,
Lu Qing,
Ding Bo,
Xu Jingyun,
Shen Yang
Publication year - 2019
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13972
Subject(s) - gper , mapk/erk pathway , signal transduction , cancer research , microbiology and biotechnology , kinase , western blot , epidermal growth factor receptor , biology , estrogen receptor , medicine , receptor , biochemistry , cancer , gene , breast cancer
Aim To study the molecular mechanism of G protein‐coupled receptor 30‐epidermal growth factor receptor (GPR30‐EGFR) signaling pathway on the proliferation of leiomyoma cells exposed with bisphenol A. Methods Primary cultures and subcultures of human uterine leiomyoma (UL) cells. The expressions of messenger RNA and proteins of GPR30 and EGFR in 15 leiomyoma tissue specimens and all groups were detected by real‐time quantitative polymerase chain reaction assay and Western blot assay. The protein of mitogen‐activated protein kinases (MAPK)/extracellular signal–regulated kinases (ERK)/c‐fos signaling pathway members was detected by Western blot assay. Results Bisphenol A promoted the growth of UL cells and the expressions of GPR30, EGFR, c‐fos and p‐ERK1/2. Conclusion Bisphenol A was found to be a promoter specifically to proliferate the human UL cells by activating the transcription and translation of GPR30‐EGFR and MAPK/ERK/c‐fos signaling pathway members.