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Ursolic acid promotes the apoptosis of cervical cancer cells by regulating endoplasmic reticulum stress
Author(s) -
Guo JinLing,
Han Teng,
Bao Le,
Li XiaoMei,
Ma JingQuan,
Tang LiPing
Publication year - 2019
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13919
Subject(s) - ursolic acid , hela , apoptosis , endoplasmic reticulum , viability assay , unfolded protein response , microbiology and biotechnology , mtt assay , cell , chemistry , biology , biochemistry , botany
Abstract Aim Ursolic acid is a triterpenoid common in plants and exhibits anti‐carcinogenic activity. This study aimed to reveal the role of endoplasmic reticulum stress in cervical cancer cell apoptosis promoted by ursolic acid. Methods HeLa cells were treated with ursolic acid or/and 4‐phenylbutyric acid. The viability and apoptosis of HeLa cells were evaluated by MTT assay and flow cytometry, respectively. Results Ursolic acid decreased HeLa cell viability in a time‐ and dose‐ dependent manner, and induced HeLa cell apoptosis in a dose‐dependent manner. Moreover, ursolic acid increased the expression of C/EBP homologous protein and glucose‐regulated protein 78 at protein levels, while 4‐phenylbutyric acid antagonized the apoptosis of HeLa cells induced by ursolic acid. Conclusion Ursolic acid inhibits the viability and promotes the apoptosis of HeLa cells. Endoplasmic reticulum stress may mediate the apoptosis of cervical cancer cells stimulated by ursolic acid.