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Detection and genomic analysis of genital group B streptococcus in pregnant Korean women
Author(s) -
Lee Hwan Tae,
Kim Suk Young,
Park PilWhan,
Ahn JeongYeal,
Kim KyungHee,
Seo Ja Young,
Jeong JiHun,
Kwoun WooJae,
Seo YielHea
Publication year - 2019
Publication title -
journal of obstetrics and gynaecology research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 50
eISSN - 1447-0756
pISSN - 1341-8076
DOI - 10.1111/jog.13810
Subject(s) - multilocus sequence typing , serotype , medicine , polymerase chain reaction , streptococcus , clindamycin , microbiology and biotechnology , streptococcus agalactiae , group b , sex organ , erythromycin , typing , virology , biology , antibiotics , bacteria , genotype , gene , genetics
Aim Group B streptococcus (GBS) is a leading cause of life‐threatening bacterial infections among newborns, and neonates born to heavily colonized women may be subject to vertical transmission. We sought to determine an appropriate detection method for genital GBS in pregnant women by comparing culture‐based methods and real‐time polymerase chain reaction (PCR). In addition, we performed molecular serotyping and multilocus sequence typing (MLST) on isolates. Methods A total of 150 pregnant women were enrolled and underwent vaginal‐rectal swabbing at 16–40 weeks of gestation. GBS was identified by conventional culture and real‐time PCR with or without enrichment. Molecular serotyping and MLST were performed on isolates. Results Overall genital GBS positive rate among the 150 study subjects was 17.3%. Direct culture identified 18 (12.0%) positive specimens, enrichment culture 22 (14.6%), direct PCR 24 (16.0%) and enrichment PCR 25 (16.6%). The sensitivity and specificity by direct and enrichment PCR were as follows: for direct PCR, 90.9% and 96.9%, respectively; and for enrichment PCR, 95.5% and 96.9%, respectively. Resistance rates to clindamycin and erythromycin were 33.3% and 19.1%, respectively. Serotype III‐1 was the most common (26.3%), followed by serotype Ib (21.1%), III‐3 (15.8%), V (15.8%), II (10.5%), IV (5.3%) and VI (5.3%). Most common sequence types (ST) were ST‐1, ST‐19 and ST‐862 (15.8%), followed by ST‐2 and ST‐654 (10.5%). Conclusion Direct real‐time PCR using vaginal‐rectal specimen could be used for detecting GBS in emergent conditions. Molecular serotypes III, Ib and V were most common. MLST analysis frequently presented ST‐1, ST‐19 and ST‐862.